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enhanced sensitive ish detection kit  (Boster Bio)


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    Boster Bio enhanced sensitive ish detection kit
    Enhanced Sensitive Ish Detection Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enhanced sensitive ish detection kit/product/Boster Bio
    Average 90 stars, based on 1 article reviews
    enhanced sensitive ish detection kit - by Bioz Stars, 2026-05
    90/100 stars

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    HFD-modulated gut microbiota regulates the expression of circRNAs. A, The circRNA expression profiles of intestinal tumors from CTL, HFD, and HFD+Abx AOM/DSS model mice were compared via high-throughput circRNA microarray sequencing. B, Mmu_circ_017841, mmu_circ_006689, mmu_circ_32011, circ_38945, mmu_circ_19191, mmu_circ_41893, and mmu_circ_45525, which appeared simultaneously in the upregulated circRNAs of the HFD vs. CTL and HFD+Abx groups. C–E , RT-qPCR was used to verify the differential expression of target mmu_circRNAs (mmu_circ_38945, mmu_circ_32011, mmu_circ_19191, and mmu_circ_45525) in groups of mice generated via three different modeling methods. F, Sanger sequencing results for circ_38945 and circ_0126925. G, Divergent and convergent primers were used to amplify circ_0126925, gDNA, and cDNA with or without RNase treatment before RT-qPCR. H, FISH analysis of the subcellular localization of circ_0126925. I, The proportions of cells in which circ_0126925 was expressed in the nucleus and cytoplasm. J and K, RT-qPCR and ISH were used to detect the expression of circ_0126925 in cancer and adjacent tissues. L, KM curve of the overall survival rate of patients with different expression levels of circ_0126925. In C–E , J , and K , unpaired and paired t tests were used for statistical analyses. In K , the differences between the KM curves were tested for significance by the log-rank test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. ns indicates no significant difference between the two indicated groups.

    Journal: Molecular Cancer Research

    Article Title: Gut Microbiota–Mediated hsa_circ_0126925 Targets BCAA Metabolic Enzyme BCAT2 to Exacerbate Colorectal Cancer Progression

    doi: 10.1158/1541-7786.MCR-24-0434

    Figure Lengend Snippet: HFD-modulated gut microbiota regulates the expression of circRNAs. A, The circRNA expression profiles of intestinal tumors from CTL, HFD, and HFD+Abx AOM/DSS model mice were compared via high-throughput circRNA microarray sequencing. B, Mmu_circ_017841, mmu_circ_006689, mmu_circ_32011, circ_38945, mmu_circ_19191, mmu_circ_41893, and mmu_circ_45525, which appeared simultaneously in the upregulated circRNAs of the HFD vs. CTL and HFD+Abx groups. C–E , RT-qPCR was used to verify the differential expression of target mmu_circRNAs (mmu_circ_38945, mmu_circ_32011, mmu_circ_19191, and mmu_circ_45525) in groups of mice generated via three different modeling methods. F, Sanger sequencing results for circ_38945 and circ_0126925. G, Divergent and convergent primers were used to amplify circ_0126925, gDNA, and cDNA with or without RNase treatment before RT-qPCR. H, FISH analysis of the subcellular localization of circ_0126925. I, The proportions of cells in which circ_0126925 was expressed in the nucleus and cytoplasm. J and K, RT-qPCR and ISH were used to detect the expression of circ_0126925 in cancer and adjacent tissues. L, KM curve of the overall survival rate of patients with different expression levels of circ_0126925. In C–E , J , and K , unpaired and paired t tests were used for statistical analyses. In K , the differences between the KM curves were tested for significance by the log-rank test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. ns indicates no significant difference between the two indicated groups.

    Article Snippet: The expression level of circ_0126925 in adjacent and cancerous tissues was detected using a Boster ISH Detection Kit (Boster Bio).

    Techniques: Expressing, High Throughput Screening Assay, Microarray, Sequencing, Quantitative RT-PCR, Quantitative Proteomics, Generated